Effect of topical mitomycin-C on total collagen deposits on the submucosa of intact vocal folds in swine.

OBJECTIVE To compare the effects of topical mitomycin-C at different concentrations on submucosal collagen deposition on the vocal folds of swine. METHODS The animals were divided into three groups according to the composition of the topical solution to be applied to the vocal folds: 0.9% saline solution (control group); 4 mg/ml mitomycin-C (group 1) and 8 mg/ml mitomycin-C (group 2). Thirty days after the application, all animals were sacrificed, their vocal folds were collected and stained by the picrosirius red technique, and submucosal collagen deposition areas were estimated by the Image Pro Plus 4.5 software. Mann-Whitney test was used to compare differences between parameters of each group. RESULTS The means of the areas of submucosal collagen deposits on vocal folds were 3110.44 square micrometers (microm(2)), 3115.98 microm(2) and 3105.78 microm(2) for groups control, 1 and 2, respectively. There were no statistical differences across the three groups (p>0.05). CONCLUSION Mitomycin-C topically applied to intact vocal folds of swine did not alter submucosal collagen deposition.